FlowJo is a powerful software widely used in the field of flow cytometry, a technique that analyzes characteristics of individual cells within a heterogeneous sample. Developed by Tree Star Inc., FlowJo facilitates the analysis and visualization of complex flow cytometry data. Researchers and scientists utilize this software to interpret multi-parameter flow cytometry experiments, helping them identify and characterize different cell populations based on fluorescence intensity and other parameters. FlowJo offers a user-friendly interface, advanced data analysis tools, and the ability to create high-quality graphics for publication. Its versatility and efficiency make it an essential tool in immunology, oncology, and various other biomedical research fields, streamlining the process of extracting valuable insights from intricate flow cytometry datasets. As an integral part of many laboratories, FlowJo continues to contribute significantly to advancements in cell biology and medical research.